Journal: European journal of nuclear medicine and molecular imaging
Article Title: Targeting MMP-14 for dual PET and fluorescence imaging of glioma in preclinical models
doi: 10.1007/s00259-019-04607-x
Figure Lengend Snippet: Human glioma patient specimens and cell lines grown in vitro express different levels of MMP-14. A MMP-14 immunofluorescence (left) and quantification of fluorescence signal (right) in a human tissue microarray (mean ± SD, n = 10–24 tissue sections/group); B MMP-14 immunofluorescence of adherent GBM cells grown in vitro and quantification of fluorescence signal (bottom right; mean ± SD, n = 25–30/group); C MMP-2 gel zymography (left) and quantification of relative active/latent MMP-2 band intensity (right) from supernatants of adherent GBM cells grown in vitro (mean ± SD, n = 3/group); scale bar in B, 20 μm; *p < 0.05; ***p < 0.001
Article Snippet: Immunohistochemistry: A slide with a formalin-fixed paraffin-embedded (FFPE) human glioma tissue microarray (BS17016b, US Biomax, Inc, Derwood, MD) was deparaffinized and antigen was retrieved by heating for 10 min at 90 °C in citrate buffer pH 6 with 1 mM EDTA.
Techniques: In Vitro, Immunofluorescence, Fluorescence, Microarray, Zymography